Latest Illumina Chemistry – Reads/Lane

In line with our efforts to democratize the latest high throughput sequencing technology, we’re pleased to announce the availability of HiSeq sequencing services with v4 chemistry. Any researcher, anywhere in the world can now order this sequencing service in a matter of minutes using Genohub.com.

The new Illumina HiSeq version 4 chemistry allows for sequencing runs with 25% greater read length (2×125 for high-output runs) and 33% more clusters.  Running two full flow cells, users can expect to generate up to 1Tb of data per week, or 167 Gb per day. At an output of 250M reads per lane, users will need at least 2 lanes to achieve ~35x coverage of the human genome. While this isn’t the most efficient tech for sequencing whole human genomes (HiSeq X Ten only requires a single lane), it certainly is for exome, transcriptome and re-sequencing applications.  Earlier this year we announced the availability of NextSeq 500 and HiSeq X Ten services on Genohub.com.

The new HiSeq v4 chemistry not only improves output, but reduces the time it takes for a sequencing run to complete. With run times taking only 6 days, we expect several of our HiSeq Rapid Run users to begin switching over to take advantage of outputs of 250M reads / lane in one week instead of 150M reads / lane in a single day. As we point this out, it’s important to note that HiSeq Rapid read lengths have also increased to 2×250. 

If you’re not sure exactly what platform, chemistry or read length is the most efficient for your application, use our Shop by Project page and enter the numbers of reads or coverage you need. We’ll display all your options!