100 Gb of Data per Day – Nextseq 500 Sequencing Services Now Available on Genohub

Nextseq 500, Genohub

Find Nextseq 500 service providers on Genohub.com

Access to the Nextseq 500, Illumina’s first high throughput desktop sequencing instrument, is now available on Genohub.com. While not the highest throughput instrument on the market, it is one of the fastest with up to a 6x increase in bases read per hour (compared to HiSeq). The instrument is ideally suited for those who need a moderate amount of sequencing data (more than a MiSeq run, less than HiSeq) in a short amount of time. We expect the highest interest to be centered around targeted sequencing (exome or custom regions) and fast RNA profiling. For exome studies, you can run between 1-12 samples in a single run and get back 4 Gb at 2 x75 or 5 Gb at a 2×100 read length. If you’re interested in RNA profiling at 10M reads per sample, you can multiplex between 12-36 samples together in a single run. A 1×75 cycle run takes as few as 11 hours to complete and 2×150 runs take ~29 hours.

You can order Nextseq 500 sequencing services today and expect to receive data back in 3-4 days ! Prices for 1 lane start at $2,250. Start your search here and use our helpful filters to narrow down your choices:  https://genohub.com/shop-by-next-gen-sequencing-technology/#query=e4c84df6f5ddd963cc48c30d3f93d505

After you’ve identified the service you need, communicate your questions directly to the service provider. We’ll make sure you get a fast response. Genohub also takes care of billing and invoicing, making domestic & international ordering a breeze. We also have an easy to use project management interface to keep communication and sample specification data in one place.

If you’re not familiar with Nextseq technology or how best this instrument can be applied to your samples, take advantage of our complementary consultation service: https://genohub.com/ngs-consultation/. We can help with your sequencing project design and make recommendation as to what sequencing service would be best suited for your experiment.

Last month we announced the availability of HiSeq X Ten services on Genohub: https://blog.genohub.com/the-1k-30x-whole-human-genome-is-now-available-for-1400/

As an efficient online market for NGS services, Genohub increases your access to the latest instrumentation and technology.  You don’t have to shell out $250K or $10M for a NextSeq or HiSeq X Ten, when access to professional services is right at your fingertips !

 

 

 

Yearly Demand for Whole Human Genome Sequencing – 400K New Genomes in 2015 ?

hgp_measures

(Figure courtesy of the National Human Genome Research Institute: http://www.genome.gov/27553526) 

Advances since the Human Genome Project ended in 2003 have been significant. With new Illumina sequencing instruments becoming operational in April, large facilities will be able to generate 18,000 whole human genomes (18,000 30x Genomes / HiSeq X Ten, a set of 10 HiSeq X Systems). As of today, these facilities include: the Broad Institute, Garvan Research Foundation, Macrogen, New York Genome Center, Novogene and WuXi PharmTech. At a rate of 1 genome / lane, this begs the question how many 30x human genomes will be sequenced in the next 3 years ? Let’s estimate that each facility will churn out around 25,000, 30x genomes/year (some of the facilities above have purchased multiple HiSeq X Tens, others have more than 10 daisy chained together). In 2015 yield from these facilities alone (assuming no one else purchased a machine) would be ~150,000 genomes. Optimistically doubling that to account for new HiSeq X Ten purchases between now and 2015 would give an estimate of ~300,000 genomes in 2015, and that’s only on the HiSeq X Ten. Assuming this year there will already be 60,000 30x (non-HiSeq X Ten) genomes sequenced, 20% growth brings this figure closer to ~400,000 genomes in 2015. While this figure certainly does not account for delays, instrument break downs, data analysis, storage and library prep bottlenecks, it represents optimistic potential for 2015.

The next question is who’s going to supply all the DNA ? Several new initiatives to sequence whole populations are quickly popping up. With £100m earmarked, the UK is planning on sequencing the genomes of up to 100,000 NHS patients by 2017 (instrument platform likely Illumina), Saudi Arabia also plans to map 100,000 of their citizens, with the Ion Proton in line ready to do all the heavy lifting: http://www.bbc.com/news/health-25216135. Craig Venter’s recent launch of the company Human Longevity plans to start sequencing 40,000 genomes with plans to “rapidly scale to 100,000 human genomes / year”: http://www.humanlongevity.com/human-longevity-inc-hli-launched-to-promote-healthy-aging-using-advances-in-genomics-and-stem-cell-therapies/.

Everything described above pertains to whole human genome sequencing and is not meant to undercut the significantly higher number of other species that will be sequenced between now and 2015. Our focus at Genohub is to make it easy for researchers interested in next generation sequencing services to access all the latest sequencing technology, including the HiSeq X Ten: https://genohub.com/shop-by-next-gen-sequencing-technology/#query=e304abac02105b87079fd1a19e70b9ed. Anyone can search for, find and order sequencing, library prep and analysis services, making owning an actual sequencing instrument not a requirement for getting access to good quality data.

 

 

BaseClear Lists Next Gen Sequencing Services On Genohub

Genohub would like to welcome BaseClear to the Genohub family. The sequencing services listed by BaseClear has added more available options for researchers on the Genohub next generation sequencing market.

BaseClear has listed the following next gen sequencing instruments and Illumina library prep options:

  • Instruments:
    • Illumina MiSeq paired end sequencing options
    • Illumina HiSeq paired end sequencing options
    • PacBio SMRT Cell
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina RNA (rRNA-depleted)

BaseClear is located in the BioSciencePark (BSP) of Leiden in the Netherlands. By using Illumina systems (HiSEQ2500 and MiSEQ) in combination with the PacBio RS system BaseClear can offer whole genome analysis and offer a wide variety of combinations of read lengths, number of tags and paired end size ranges. The lab has been ISO17025 accredited since 2006.

We are excited to include BaseClear’s high throughput sequencing services on the Genohub next gen sequencing market.

Micro-Seq Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome Micro-Seq Enterprises to the Genohub family. The sequencing services listed by Micro-Seq has added more available options for researchers on the Genohub next generation sequencing market.

Micro-Seq listed the following next generation sequencing instrument and Illumina library preparation options:

  • Instrument:
    • Illumina MiSeq paired end sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina Small RNA (microRNA)
    • Illumina RNA (rRNA-depleted)
    • Illumina Bisulfite

Micro-Seq Enterprises was formed to help reduce the sometimes long delay researchers are forced to endure to get the results of their experiments that involve DNA or RNA sequencing (RNA-seq, RNAseq), including bacterial community analysis (16S sequencing). Micro-Seq is able to offer quick turnaround times for researchers with smaller sequencing projects. Unlike sequencers with enormous capacity, the economic scale of the Illumina MiSeq prevents researchers with smaller sequencing projects from enduring longer wait times and higher fees.

 

We are excited to include Micro-Seq’s high throughput sequencing services on the Genohub next generation sequencing market.

Global Biologics Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome Global Biologics to the Genohub family. The sequencing services listed by Global Biologics has added more available options for researchers on the Genohub next generation sequencing market.

Global Biologics listed the following next generation sequencing instrument and Illumina library preparation options:

  • Instrument:
    • Illumina HiSeq paired end sequencing and single read sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina DNA Hydroxymethylation
    • Illumina ChIP
    • Illumina Small RNA (microRNA)
    • Illumina Directional RNA (polyA-selected)
    • Illumina Bisulfite
    • Illumina Mate Pair (7-15 kb)

Global Biologics is a research & development service laboratory focused on clients performing discovery and applied research from academic, government, & private organizations. With experienced personnel and efficient molecular services, Global Biologics can aid scientists in advancing complex research studies.

The nucleic acid services offered by Global Biologics address common laboratory bottlenecks which are critical yet time consuming to perform. Specifically, Global Biologics has expertise in the following areas:

  • NGS Library construction & Sequencing (Illumina)
  • PCR based gene expression profiling and genotyping
  • Targeted Sequencing Applications (HiSeq) & Data Analysis
  • Nucleic Acid Isolation, QC and archive
  • Automation, scale up, and tech transfer

We are excited to include Global Biologic’s high throughput sequencing services on the Genohub instant matching engine.

Wyzer Biosciences Lists Services On the Genohub Next Generation Sequencing Market

Genohub would like to welcome Wyzer Biosciences to Genohub. The sequencing services listed by Wyzer will add more available options to the Genohub next generation sequencing market.

Wyzer listed next generation sequencing services for an Ion PGM 314 Chip.

Privately held, Wyzer Biosciences, Inc. was founded in 2011 and is located in dynamic Cambridge, MA, the home of Harvard, MIT and many other world-class educational and biotech institutions. Through their expertise in molecular biology they provide services to partners and collaborators in industry and academia that will aid them in their ongoing research. We look forward to matching Wyzer with the most compatible high throughput sequencing projects for their lab.

Gnomegen Lists Services On the Genohub Next Generation Sequencing Market

We want to welcome Gnomegen to the Genohub family. The sequencing services listed by Gnomegen will add more available options to the Genohub next generation sequencing market.

Gnomegen listed the following next generation sequencing instruments and library prep options:

  • Instruments:
    • Illumina HiSeq paired end sequencing and single read sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina Directional RNA (polyA-selected)
    • Illumina ChIP
    • Illumina Small RNA (microRNA)
    • Illumina Amplicon
    • Illumina RNA (polyA-selected)
    • Illumina Total RNA
    • Ion RNA (polyA-selected)
    • Ion ChIP
    • Ion Small RNA (microRNA)
    • Ion Total RNA
    • Ion Directional RNA (polyA-selected)
    • Roche454 DNA

Being an early stage startup, Gnomegen has successfully developed and commercialized reagent products for the library preparation of next generation sequencing with pending patents on file. Besides its team of creative scientists, Gnomegen is backed up by consultants with leading expertise in technology, sales and marketing as well as corporate attorneys and patent agents. We look forward to matching Gnomegen with the most compatible high throughput sequencing projects for their lab.