We often get questions from researchers on selecting the best next generation sequencing platforms for specific projects. We are glad to offer free consultation to researchers on inquiries such as these among others. We also offer a sequencing guide to get you started.
Another great resource is a YouTube talk by Dr Elaine Mardis, a professor of Genetics and Molecular Biology and the co-director of the Genome Institute at the Washington University School of Medicine. She begins by discussing next gen sequencing instrument similarities, such as library amplification, nucleotide detection, read lengths, and paired end sequencing. She then dives into the factors unique to individual next gen sequencing instruments by Roche 454, Life Technologies, Illumina, Ion Torrent, PacBio, and Oxford Nanopore. The discussion focuses on the benefits and dissimilarities among them associated with:
- Paired end reads: linear vs circularized fragments
- Sequencing technologies: DNA polymerase, DNA ligase, synthesis H+ detection, syntheses, and nanopore
- Library amplification methods: emPCR, bridge amplification, and its absence in some 3rd gen platforms
- Run times
- Error rates
- Read lengths
Below, you can view the video in its entirety which goes into quite some detail and discusses the best types of projects for each NGS platform.