BaseClear Lists Next Gen Sequencing Services On Genohub

Genohub would like to welcome BaseClear to the Genohub family. The sequencing services listed by BaseClear has added more available options for researchers on the Genohub next generation sequencing market.

BaseClear has listed the following next gen sequencing instruments and Illumina library prep options:

  • Instruments:
    • Illumina MiSeq paired end sequencing options
    • Illumina HiSeq paired end sequencing options
    • PacBio SMRT Cell
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina RNA (rRNA-depleted)

BaseClear is located in the BioSciencePark (BSP) of Leiden in the Netherlands. By using Illumina systems (HiSEQ2500 and MiSEQ) in combination with the PacBio RS system BaseClear can offer whole genome analysis and offer a wide variety of combinations of read lengths, number of tags and paired end size ranges. The lab has been ISO17025 accredited since 2006.

We are excited to include BaseClear’s high throughput sequencing services on the Genohub next gen sequencing market.

UTHSCSA Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome the Genome Sequencing Facility (GSF) at Greehey Children’s Cancer Research Institute in the University of Texas Health Science Center at San Antonio to the Genohub family. The sequencing services listed by UTHSCSA has added more available options for researchers on the Genohub next generation sequencing market.

UTHSCSA listed the following next generation sequencing instruments and Illumina library preparation options:

  • Instruments:
    • Illumina MiSeq paired end sequencing options
    • Illumina HiSeq single read and paired end sequencing options
  • Library prep:
    • Illumina ChIP
    • Illumina Directional RNA (polyA-selected)
    • Illumina Directional RNA (rRNA-depleted)
    • Illumina DNA
    • Illumina Exome
    • Illumina RNA (polyA-selected)
    • Illumina RNA (rRNA-depleted)
    • Illumina Small RNA (microRNA)

The GSF has a stated mission to deploy NGS systems focusing on cancer genomics to benefit Greehey CCRI investigators and the wider research community. The NGS facility performs all protocols necessary for preparing a sample to be sequenced on the Illumina HiSeq 2000 system. The GSF is also a research group with experience in customizing experiments and developing new applications. They provide:

  • Complete project consultation for optimal experimental design and setup
  • Complete quality control analysis during library preparation and data generation
  • Secure website to download the sequencing data with quality information
  • Full project report
  • Bioinformatics analysis option available
  • NCBI GenBank submission option available

We are excited to include UTHSCSA high throughput sequencing services on the Genohub next generation sequencing market.

How to Select the Best Next Generation Sequencing Platform For Your Project

We often get questions from researchers on selecting the best next generation sequencing platforms for specific projects. We are glad to offer free consultation to researchers on inquiries such as these among others. We also offer a sequencing guide to get you started.

Another great resource is a YouTube talk by Dr Elaine Mardis, a professor of Genetics and Molecular Biology and the co-director of the Genome Institute at the Washington University School of Medicine. She begins by discussing next gen sequencing instrument similarities, such as library amplification, nucleotide detection, read lengths, and paired end sequencing. She then dives into the factors unique to individual next gen sequencing instruments by Roche 454, Life Technologies, Illumina, Ion Torrent, PacBio, and Oxford Nanopore. The discussion focuses on the benefits and dissimilarities among them associated with:

  • Paired end reads: linear vs circularized fragments
  • Sequencing technologies: DNA polymerase, DNA ligase, synthesis H+ detection, syntheses, and nanopore
  • Library amplification methods: emPCR, bridge amplification, and its absence in some 3rd gen platforms
  • Run times
  • Error rates
  • Read lengths

Below, you can view the video in its entirety which goes into quite some detail and discusses the best types of projects for each NGS platform.

Micro-Seq Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome Micro-Seq Enterprises to the Genohub family. The sequencing services listed by Micro-Seq has added more available options for researchers on the Genohub next generation sequencing market.

Micro-Seq listed the following next generation sequencing instrument and Illumina library preparation options:

  • Instrument:
    • Illumina MiSeq paired end sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina Small RNA (microRNA)
    • Illumina RNA (rRNA-depleted)
    • Illumina Bisulfite

Micro-Seq Enterprises was formed to help reduce the sometimes long delay researchers are forced to endure to get the results of their experiments that involve DNA or RNA sequencing (RNA-seq, RNAseq), including bacterial community analysis (16S sequencing). Micro-Seq is able to offer quick turnaround times for researchers with smaller sequencing projects. Unlike sequencers with enormous capacity, the economic scale of the Illumina MiSeq prevents researchers with smaller sequencing projects from enduring longer wait times and higher fees.

 

We are excited to include Micro-Seq’s high throughput sequencing services on the Genohub next generation sequencing market.