8 Top Online Calculators for Next Gen Sequencing

When it comes to next gen sequencing, here are the top 8 online calculators that we have found:

Coverage

  1. Genohub’s shop by project feature
  2. The Coverage Calculator by Clinical Genomics

Oligonucleotide

  1. The Oligo Calc: Oligonucleotide Properties Calculator by Northwestern University
  2. The OligoAnalyzer 3.1 by Integrated DNA Technologies

Primers

  1. NCBI/ Primer-BLAST: Finding primers specific to your PCR template (using Primer3 and BLAST) 
  2. Primer3: WWW primer tool by UmassMed

qPCR Efficiency

  1. The qPCR efficiency calculator by Thermo Scientific
  2. The QPCR Standard Curve Slope to Efficiency Calculator by Agilent Technologies

If you know of any other great online calculators for next gen sequencing please tell us about it!

BaseClear Lists Next Gen Sequencing Services On Genohub

Genohub would like to welcome BaseClear to the Genohub family. The sequencing services listed by BaseClear has added more available options for researchers on the Genohub next generation sequencing market.

BaseClear has listed the following next gen sequencing instruments and Illumina library prep options:

  • Instruments:
    • Illumina MiSeq paired end sequencing options
    • Illumina HiSeq paired end sequencing options
    • PacBio SMRT Cell
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina RNA (rRNA-depleted)

BaseClear is located in the BioSciencePark (BSP) of Leiden in the Netherlands. By using Illumina systems (HiSEQ2500 and MiSEQ) in combination with the PacBio RS system BaseClear can offer whole genome analysis and offer a wide variety of combinations of read lengths, number of tags and paired end size ranges. The lab has been ISO17025 accredited since 2006.

We are excited to include BaseClear’s high throughput sequencing services on the Genohub next gen sequencing market.

UTHSCSA Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome the Genome Sequencing Facility (GSF) at Greehey Children’s Cancer Research Institute in the University of Texas Health Science Center at San Antonio to the Genohub family. The sequencing services listed by UTHSCSA has added more available options for researchers on the Genohub next generation sequencing market.

UTHSCSA listed the following next generation sequencing instruments and Illumina library preparation options:

  • Instruments:
    • Illumina MiSeq paired end sequencing options
    • Illumina HiSeq single read and paired end sequencing options
  • Library prep:
    • Illumina ChIP
    • Illumina Directional RNA (polyA-selected)
    • Illumina Directional RNA (rRNA-depleted)
    • Illumina DNA
    • Illumina Exome
    • Illumina RNA (polyA-selected)
    • Illumina RNA (rRNA-depleted)
    • Illumina Small RNA (microRNA)

The GSF has a stated mission to deploy NGS systems focusing on cancer genomics to benefit Greehey CCRI investigators and the wider research community. The NGS facility performs all protocols necessary for preparing a sample to be sequenced on the Illumina HiSeq 2000 system. The GSF is also a research group with experience in customizing experiments and developing new applications. They provide:

  • Complete project consultation for optimal experimental design and setup
  • Complete quality control analysis during library preparation and data generation
  • Secure website to download the sequencing data with quality information
  • Full project report
  • Bioinformatics analysis option available
  • NCBI GenBank submission option available

We are excited to include UTHSCSA high throughput sequencing services on the Genohub next generation sequencing market.

Micro-Seq Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome Micro-Seq Enterprises to the Genohub family. The sequencing services listed by Micro-Seq has added more available options for researchers on the Genohub next generation sequencing market.

Micro-Seq listed the following next generation sequencing instrument and Illumina library preparation options:

  • Instrument:
    • Illumina MiSeq paired end sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina 16S V4
    • Illumina Small RNA (microRNA)
    • Illumina RNA (rRNA-depleted)
    • Illumina Bisulfite

Micro-Seq Enterprises was formed to help reduce the sometimes long delay researchers are forced to endure to get the results of their experiments that involve DNA or RNA sequencing (RNA-seq, RNAseq), including bacterial community analysis (16S sequencing). Micro-Seq is able to offer quick turnaround times for researchers with smaller sequencing projects. Unlike sequencers with enormous capacity, the economic scale of the Illumina MiSeq prevents researchers with smaller sequencing projects from enduring longer wait times and higher fees.

 

We are excited to include Micro-Seq’s high throughput sequencing services on the Genohub next generation sequencing market.

Press Release: Genohub Launches Next Generation Sequencing Marketplace

You can view the original press release on our official launch at PRWeb.com. Thanks to the folks at RNA-Seq Blog for quickly picking up the story.

Genohub announced the launch of their online market for next generation sequencing services today.

Austin, TX (PRWEB) August 16, 2013

Genohub.com announced the launch of their online market for next generation sequencing services today. The online service, is positioned to completely change the way high throughput sequencing services are ordered, accelerating genomic research by improving access to sequencing services. Genohub’s intelligent sequencing matching engine instantly matches researchers with service providers based on specific project criteria. Genohub facilitates the management of sequencing projects throughout the sequencing lifecycle from selecting orderable sequencing packages, to communication, payments and delivery of data.

For Researchers

Genohub’s online service transforms the way researchers go about ordering next generation sequencing (NGS) and reinforces the critical researcher-provider communication cycle involved in every project. Genohub’s model eliminates the need for researchers to call multiple service providers to compare service details and prices. Researchers use the smart NGS matching engine to gain immediate access to up-to-date service listings from reputable providers. The transparent pricing model, with exact service prices, reduces the time needed to compare services and makes it significantly faster, more informed and more accurate than manually ordering by email or phone. Researchers using the service are also able to take advantage of one-time deals and other offers not normally available through a provider’s website or pricing sheet. Clear maximum turnaround times for each service reduces the unpredictability associated with project completion dates. Researchers using the service are able to track the status of their orders, upload data or project specific information and post messages to providers performing the work.

Genohub’s shopping interface is designed to accommodate both researchers with prior experience with the latest sequencing technology, as well as the increasing number of life science researchers who are not necessarily familiar with the latest sequencer specs or perhaps have no prior sequencing experience at all. Experienced users can search by selecting specific instruments and run types while researchers new to sequencing can shop for services by their project requirements, e.g., read number and coverage. Researchers who need help selecting the right sequencing service can also take advantage of free consultation by Genohub’s PhD trained staff.

For Service Providers

Genohub has also invested significantly on promoting NGS service providers by allowing them to advertise services and extend their reach to places where they would normally not receive orders. Providers sign up and list their services in a structured format allowing Genohub’s matching engine to automatically offer services to customers based on their experimental needs. The online service facilitates customer communication via a centralized messaging interface, which allows providers to request data, convey unforeseen handling or quality issues and relay project status to the researcher.

Genohub also automatically generates accurate quotes based on the pricing information that has to be entered by the provider only once. This significantly reduces the amount of time providers would normally have to spend on creating and communicating quotes

“While high-throughput sequencing holds enormous potential for unlocking new discoveries, the high cost and complexity of sequencing projects necessitate a professional marketplace like Genohub to improve access and facilitate collaboration between researchers and service providers across universities, companies, as well as other private and public research organizations around the globe,” said Pouya Razavi, Genohub’s CEO and co-founder.

Media Contact:
Estevan McCalley, Head of Customer Development
Genohub
512-436-0111
info@genohub.com
https://genohub.com/

Global Biologics Lists Next Generation Sequencing Services On Genohub

Genohub would like to welcome Global Biologics to the Genohub family. The sequencing services listed by Global Biologics has added more available options for researchers on the Genohub next generation sequencing market.

Global Biologics listed the following next generation sequencing instrument and Illumina library preparation options:

  • Instrument:
    • Illumina HiSeq paired end sequencing and single read sequencing options
  • Library prep:
    • Illumina DNA
    • Illumina DNA Hydroxymethylation
    • Illumina ChIP
    • Illumina Small RNA (microRNA)
    • Illumina Directional RNA (polyA-selected)
    • Illumina Bisulfite
    • Illumina Mate Pair (7-15 kb)

Global Biologics is a research & development service laboratory focused on clients performing discovery and applied research from academic, government, & private organizations. With experienced personnel and efficient molecular services, Global Biologics can aid scientists in advancing complex research studies.

The nucleic acid services offered by Global Biologics address common laboratory bottlenecks which are critical yet time consuming to perform. Specifically, Global Biologics has expertise in the following areas:

  • NGS Library construction & Sequencing (Illumina)
  • PCR based gene expression profiling and genotyping
  • Targeted Sequencing Applications (HiSeq) & Data Analysis
  • Nucleic Acid Isolation, QC and archive
  • Automation, scale up, and tech transfer

We are excited to include Global Biologic’s high throughput sequencing services on the Genohub instant matching engine.

The Institute for Personalized and Predictive Medicine of Cancer (IMPPC) Lists High Throughput Sequencing Services On the Genohub Market

We want to welcome The Institute for Personalized and Predictive Medicine of Cancer (IMPPC) to the Genohub family. The high throughput sequencing services listed by IMPPC will add more available options to the Genohub next generation sequencing market.

IMPCC listed the following next generation sequencing instruments and library prep options:

  • Instruments:
    • Illumina HiScanSQ
    • Ion PGM 314 Chip
    • Ion PGM 316 Chip
    • Ion PGM 318 Chip
  • Library prep:
    • Illumina Directional RNA (polyA-selected)
    • Illumina Small RNA (microRNA)
    • Ion DNA

The IMPCC is a publicly funded institute backed by a private foundation with a mission to advance in the understanding of genetic and epigenetic factors leading to higher predisposition or susceptibility to develop cancer. We look forward to matching IMPPC with the most compatible high throughput sequencing projects for their lab.